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To Do
Lab work (Can do)(Waiting on parts)(Dependent on other process)ExperimentsPlanning
  •  Nanodrop Syk+Lyn
    •  Sequence Syk+Lyn
    •  verify sequence
  •  PCR fusion proteins
  •  Golden Gate fusion proteins
  •  multiply fusion protein
    •  Put in cells and plate
    •  pick colonies
    •  miniprep and make frozen stock
    •  sequence
    •  verify sequence
  •  Multiply/make antibody stock
    •  plate stab cultures
    •  pick colonies
    •  miniprep and freeze
    •  sequence
    •  verify sequence
  •  Check BCR localization
  •  Check IgM Binding
  •  Check Lyn Localization
  •  Check Syk Localization
  •  Characterize Syk-TEVp cleavage
 

Project Planning Link

Cloning Strategies

Experimental Overview

Proteins needed

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