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pENTR L1-Gene name-L2

pEXPR Promoter:Gene

NamePriority
NAMESTATUSModuleTYPEStatusLOCATIONTypeINITIALSInitialsConstruction DateLinkExperimentCONSTRUCTED ONOBTAINED FROMPRIORITYOTHER
Antibody Group
pEXPR Gal4UAS:eYFPantibodyin someone else's freezerexpression      
pEXPR Gal4UAS:mKateantibodyin someone else's freezer

expression

      
pEXPR Tre-TEVpantibodyin someone else's freezerexpression      
Hef1a-rtTaantibodyin someone else's freezerexpression      
CD79A WTantibodyminiprepped, awaiting sequence verification       
CD79B WTantibodyminiprepped, awaiting sequence verification       
CD79A-TCS-Gal4VP16antibodyplannedplanned       
CD79B-TCS-Gal4VP16antibodyplannedplanned       
Lyn WTantibodygrowing in LB, ready at 7am       
Lyn-eYFPantibodyplannedplanned       
Lyn point mutated (always phosphorylate)antibodyresearchingresearching       

Syk WT

antibody

growing in LB, ready at 7am       
Syk-eYFP (mutant non phosphorylate)antibodyplannedplanned       
Syk-TEVpantibodyplannedplanned       
GMab kantibodyon order       
GMab Hantibodyon order       
GMabH-TCS-Gal4VP16antibodyplannedplanned       
miRNA Group
hsa-miR-181cmiRNAplannedplanned       
hsa-miR-9miRNAplannedplanned       
hsa-miR-30d-5pmiRNAplannedplanned       
hsa-miR-144-5pmiRNAplannedplanned       
hsa-let-7f-5pmiRNAplannedplanned       
has-miR-125bmiRNAplannedplanned       
has-miR-146amiRNAplannedplanned       
Treatment Group
pEXPR TRE-t:BACE2-eYFPtreatmentplannedplanned       
    • pENTR L4--TRE-t--R1
treatment
in the lab already       
    • pENTR L1--BACE2-eYFP--L2
treatmentplannedplanned       
treatment
in -80plasmid AL    
      • eYFP
treatmentin the lab already       
    • pDEST R4--R2
treatmentin the lab already       
pEXPR Hef1a:BACE1-eYFPtreatmentplannedplanned       
    • pENTR L4--Hef1a--R1
treatment
in the lab already       
    • pENTR L1--BACE1-eYFP--L2
treatmentplannedplanned       
      • BACE1
treatmentto be ordered       
      • eYFP
treatmentin the lab already       
    • pDEST R4--R2
treatment
in the lab already       
pEXPR U6:mKate-miRNA(intronic)treatmentplannedplanned       
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)treatmentplanned       
Receptor Group
Experiment 1        
  • ggDONR
were not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enoughplasmid+4 in iGEM roomLA- Kyle  
  • ggDONR New
will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stockplasmid+4 in iGEM roomLA-Brandon  
  • Hef1a Entry Vector
in planning - will be used to make LilrB2 expression vectorplasmid      
  • Destination Vector
in planning - will be used to make LilrB2 expression vectorplasmid      
  • LirB2 Expression Vector
in construction - waiting on entry vectorplasmid      
    • LilrB2 Entry Vector
in construction - failing to get liquid cultures to grow in order to miniprep to extract plasmid DNAplasmid      
      • LilrB2 gBlock
used in Golden Gate - in storagegBlock-20 in iGEM roomLA- IDT  
  • PirB Expression Vector
in construction - waiting on entry vector       
    • PirB Entry Vector
in construction - failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA       
      • PirB gBlock C terminus
used in Golden Gate - in storagegBlock-20 in iGEM roomLA-IDT  
      • PirB gBlock N terminus
used in Golden Gate - in storage gBlock-20 in iGEM roomLA-IDT  
         
         
   planned