...
Progress:
To Do:
- Making pEXPR for LilrB2 and PirB
- Miniprep liquid culture
- LR into pDEST
- find out if there is enough for a PCR for the fusions (if not - pick new colonies)
- Troubleshooting ggDONR stock
- run gel with ggDONR
- ask to use someone's working ggDONR
- Making pEXPR for LilrB2/PirB - BFP fusions
- PCR
- Golden gate
- Transfect and plate
- Pick colonies
- Miniprep
- LR
...