| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Email sent | ||
Jeremy Gam | Questions | Had first meeting |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
Presentations
- Run through NEGEM Presentation (12:00)
miRNA Sensors (Theory)
- Create circuit diagram (in the NEGEM presentation?)
- Put it on our subgroup home page
- How does repressor affect the circuit?
- Determine final output of circuit
miRNA Sensors (Lab Work)
- Understand why we're doing any of these reactions
- Transform MAV1212 into ccdB resistance cells
- Grow at low temp w/ x-gal, shielded from light
- Pick as many colonies as possible
- Sequence ccdB region
- Propogate plasmids (in room 215, in -20 fridge, labeled "igem 2014")
- MAV1212
- MAV1212-Hef1a-EBFP2
- MAV1212-Hef1a-mKate2
- Gateway MAV1212-Hef1a-L7ae
- Find our repressors and repressible promoters promoters
- Find entry vectors in our library
Cell Models
- Finish table of concentrations in each cell line
- Finalize siRNA transfection technique