| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Email sent | ||
Jeremy Gam | Questions | Meeting Sunday/Monday |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
Lab contacts
- Email our contacts
- Finalize list of questions for our contacts
miRNA
- Finish miRNA profile explanations
- Pathways it's involved in
- How up/down regulated is it? (T Test)
- List of cells we have access to
- Email Samira about what she just ordered
- Break down sensor assembly plan (from Brian)
- Start drafting preliminary experiments
Presentation
- Divide labor for next week's presentation slides
What to do, what to do...
Expression levels of each miRNA in profile in each cell lineNowhere to be found on the internet. If we want to know it, we're going to have to do the assays ourselves (as a mini experiment)miRNA Sensors
- Put in order for miRNA and other parts
- Write out detailed plan for cloning and Gibson/Gateway reactions
- What we're doing
- Why we're doing it
- What we need
- Determine repressor for circuit
Cell Models
- Finish table of concentrations in each cell line
- Finalize siRNA transfection technique