...
- Remove GGDonr liq culture from incubator (9am, Lyla)
- Make cell stock, store at -80 degrees
- Put rest of cell culture in 4 degrees
- Remove pENTR cultures from incubator (10:30 am30am, Shinjini)
- Pick cells from pENTR colonies, put in liquid culture, put in incubator at 6pm (16 hours)
Sunday:
- Take pENTR colonies from incubator. Make cell stock. Put in fridge and freezer respectively. (10 am)
Monday:
- Make empty liquid cell culture to transform DNA into (~16hours)
- Make 5 femptomolal working solution
- LR (~ 16 hours min, 24 max)
- Miniprep GGDonr
- PCR (possibly)
...