...
- Nanodrop pENTR (was not useful)
- Transform pENTR into bacteria
- Incubate over night
- (5pm) Add Kan, incubate GGDonr liq culture (16hrs)
Saturday:
- Remove GGDonr liq culture from incubator (9am, Lyla)
- Make cell stock, store at -80 degrees
- Put rest of cell culture in 4 degrees
- Remove pENTR cultures from incubator (10:30 am, Shinjini)
- Pick cells from pENTR colonies, put in liquid culture, put in incubator (16 hours)
...