ToDo:
Friday:
- Nanodrop pENTR (was not useful)
- Transform pENTR into bacteria
- Incubate over night
- (5pm) Add Kan, incubate GGDonr liq culture (16hrs)
...
- Remove GGDonr liq culture from incubator (9am, Lyla)
- Make cell stock, store at -80 degrees
- Put rest of cell culture in 4 degrees
- Remove pENTR cultures from incubator (10:30 am, Shinjini)
- Pick cells from pENTR colonies (when?) , put in liquid culture, put in incubator (16 hours)
...
- Make empty liquid cell culture to transform DNA into (~16hours)
- Make 5 femptomolal working solution
- LR (~ 16 hours min, 24 max) Monday
- Miniprep GGDonr
- PCR (possibly)
...