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Making LilrB2/PirB expression vectorsStart DateProgress/condition/next stepsCompleted? (Date)ResultLocation of part (if needed, I don't know)

Making pENTR from gBlocks:

We have:
Q1_LilrB2_Qx
Q1_PirB-N_Q2, Q2_PirB-C_Qx

We need:
pGGDONR : has Q1 and Qx sites, L1 and L2 sites

Golden-gating will result in:
pENTR_L1_LilrB2_L2 (order of L's correct, I checked!)
pENTR_L1_PirB2_L2

6/12  GG mixtures in thermocycler

GGDonr cell (culture?) in incubator

Will make GGDonr bacterial stock today (6/13)

   fridge (in case transformations did not work)

After that, Gateway (LR):
pENTR_L1_LilrB2_L2
pENTR_L4_Hef1a_R1 (not sure if this is the one we want)
pZDonor 1-GTW-2 (this is the DEST Shinjini used in her Geneious)

Same for PirB
pENTR_L1_PirB_L2
pENTR_L4_Hef1a_R1
pZDonor 1-GTW-2

We will get:

12_Hef1a_LilrB2
12_Hef1a_PirB

    


6/12/14

GoldenGate table:

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