...
- Remove GGDonr liq culture from incubator (9am)
- Make cell stock, store at -80 degrees
- Put rest of cell culture in 4 degrees
- Pick cells from pENTR colonies (when?) put in liquid culture, put in incubator (16 hours)
...
Sunday:
- Take pENTR colonies from incubator. Make cell stock. Put in fridge and freezer respectively.
Monday:
- Make empty liquid cell culture to transform DNA into (~16hours)
- Make 5 femptomolal working solution
- LR (~ 16 hours min, 24 max) Monday
- Miniprep GGDonr
- PCR (possibly)
...