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ToDo:

Friday:

  •  Nanodrop pENTR
  •  Make 5 femptomolal working solution
  •  Add Kan, incubate GGDonr liq culture (16hrs)

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Saturday:

  •  Remove GGDonr liq culture from incubator
  •  Make cell stock, store at -80 degrees
  •  Put rest of cell culture in 4 degrees

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Monday:

  •  Make empty liquid cell culture to transform DNA into (~16hours)
  •  LR (~ 16 hours min, 24 max) Monday
  •  Miniprep GGDonr
  •  PCR (possibly)

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June 9

Plan of Action

 - Build hef1+lilrb2

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