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Thermocycler:

protocol EBGG


37C for 5min

Part 1
50X:
37C for 2.5min
4C for 0.5min
16C for 5.5min

Part 2
37C for 10 min
80C for 20 min

4C hold

takes 8+ hours


(check protocol by looking up the paper or other online GG protocols)