...
| Add | Conc | Volm | Order of adding | ||
| DNA1 | c1 ng/ul | x1 = 50/c1 ul (50ng) | 2 | ||
| ... | ... | ... | ... | ||
| DNAn | cn | DNA2 | c2 ng/ul | x2 xn = 50/c2 cn ul (50ng) | 2 |
| GGDonr | c3 d ng/ul | y = 50/c3 d ul (100ng) | 2 | ||
| 10x T4 Ligase Buffer | 2ul | 3 | |||
| BSA | 2ul | 3 | |||
| BsaI (enzyme) | 1ul | 4 | |||
| T4 Ligase (enzyme) | 1ul | 3 | |||
| H20 | 20 - (20 - x1 - x2x1 + ... + xn + y) ul | 1 | |||
| Total | 20ul |
Take a p20, set it to 10uL and then pipet up and down.
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