Versions Compared

Old Version 7

changes.mady.by.user Unknown User (shinjini@mit.edu)

Saved on

compared with

New Version 8

changes.mady.by.user Unknown User (shinjini@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Making LilrB2/PirB expression vectorsStart DateProgress/condition/next stepsCompleted? (Date)ResultLocation of part (if needed, I don't know)

Making pENTR from gBlocks:

We have:
Q1_LilrB2_Qx
Q1_PirB-N_Q2, Q2_PirB-C_Qx

We need:
pGGDONR : has Q1 and Qx sites, L1 and L2 sites

Golden-gating will result in:
pENTR_L1_LilrB2_L2 (order of L's correct, I checked!)
pENTR_L1_PirB2_L2

 6/12

GG mixtures in thermocycler

GGDonr cell (culture?) in incubator

Will make GGDonr Bacterial stock today (6/13)

 

   

After that, Gibson (LR):
pENTR_L1_LilrB2_L2
pENTR_L4_Hef1a_R1 (not sure if this is the one we want)
pZDonor 1-GTW-2 (this is the DEST Shinjini used in her Geneious)

Same for PirB
pENTR_L1_PirB_L2
pENTR_L4_Hef1a_R1
pZDonor 1-GTW-2

We will get:

12_Hef1a_LilrB2
12_Hef1a_PirB

     

...