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x1 uL of DNA1
x2 uL of DNA2
...
y uL (100ng) Donor
2ul 10X T4 Ligase Buffer
2ul 10X BSA
1ul BsaI -HF(enzyme)
1ul T4 Ligase HC(enzyme)


fill to 20uL with SDIH20 (put water in before the buffer and enzymes)
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20ul total

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