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- "In general, we target the coding region; however, targeting the 5′- and 3′-UTR sequences is possible"
- "It is important to note that siRNA design rules serve more as guidelines, and that sequences adhering to them may not silence and vice versa. To date, no algorithm guarantees silencing efficacy, and most recommend the user pick three to four candidates for screening."
- "To achieve faithful loading of the antisense strand, the duplex must be designed such that there is strong G–C base pairing present at the 5′-end of the sense (passenger) strand and weak A/G-U base paring at the opposing terminus"
Questions/concerns about miRNA:
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