Versions Compared

Old Version 2

changes.mady.by.user Unknown User (shinjini@mit.edu)

Saved on

compared with

New Version 3

changes.mady.by.user Unknown User (shinjini@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

Fusion proteins:

 

Fixing frameshifts:

(NNN)(NNN)(NNN)(GGGG)(ATG)(NNN)(NNN)(NNN)

  protein1                    Q site             protein2

 

Add nucleotides:

(NNN)(NNN)(NNN)(TWO nucleotides)(GGGG)(ATG)(NNN)(NNN)(NNN)

  protein1                                                       Q site             protein2

OR

(NNN)(NNN)(NNN)(GGGG)(TWO nucleotides)(ATG)(NNN)(NNN)(NNN)

  protein1                    Q site                                                  protein2

 

 

PirB - goldengate the two pieces into a pENTR before PCRing the linkers on.