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Proteins needed - CD79A
- normal
- fusion: CD79A C terminus + tev cut site + FP
- CD79B
- normal
- fusion: CD79B C terminus + tev cut site + FP
- Lyn
- normal
- fusion: FP tagged (N or C terminus?)
- point mutation: mutated to always phosphorylate
- Syk
- fusion, point mutation: FP attached(N or C terminus?), prevent phosphorylation (FP tagged SYK shouldn't be in same cell as FP anything else, ought be no cross talk)
- fusion, point mutatino: tev protease attached (N or C terminus?), point mutated to prevent phosphorylation
- ig k
- with proper localization tag
- igM heavy
- with proper constant region for membrane localization and proper localization tag
we have eBFP(2?), eYFP, mKATE - Antibody that targets membrane bound igM that is dyed so we can see it
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