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| Possible Results | Possible Explanation | Controls | Protocols | Parts |
|---|---|---|---|---|
YFP visible on surface | success | Image cell with out Dox. No fluorescence should be present | PCR | Primers (AL1-4), BACE2 plasmid, eYFP plasmid |
| YFP visible in the cell | YFP not bound to BACE, YFP disrupts BACE signal sequence | GoldenGate (digest and run on gel to confirm proper assembly) | PCR products, GGDonr | |
| YFP visible out of cell | YFP bound to incorrect terminal of BACE | LR (digest and run on gel to confirm proper assembly) | GG product, TRE-t, backbone | |
| No YFP visible | no Dox, cloning mistake | Transfect | LR product, rTTA vector | |
| Assay Bace2 activity | ||||
| Image |
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