...
Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
| Experiment | Expected Results | Controls | Protocols | Parts | Completed? |
|---|---|---|---|---|---|
| Assay down-regulation of BACE1 by U6/miRNA | |||||
| Assay transcription factor's effectiveness in promoting expression of miRNA/BACE2 | |||||
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Appendix
data sets
https://www.addgene.org/26736/ (mouse BACE1 for $65)
http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236 (human BACE1; pricing given only to registered customers)