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In our proposed detection model, we would construct a fusion protein where a linker, TEV protease cleavage site and transcription factor (in that order) are fused to the intracellular domain of LilrB2. We would also fuse a TEV protease to cofilin. In that way, when beta-amyloid oligomers bind to the receptor, thus activating it, the TEV protease on the recruited cofilin cleaves at the cleavage site releasing the transcription factor in to the cytosol. The cleaved portion will be guided to the nucleus of the cell and will go on to activate the subsequent processing module.
Experiments:
| Experiment | Expected Results | Controls | Protocols | Parts | Completed? |
|---|---|---|---|---|---|
| |||||
- Making beta-oligomers and testing the selectivity of the receptors to beta-amyloid oligomers vs beta-amyloid monomers