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1. Image the gel
2. Cut the extraction bands under UV (to see the bands)
3. Follow protocol

04/20/14

Extraction from gel (similar to miniprep):

--> Weight

1. 106mg
2. 96mg

--> Add 3x vol of QG

1. 318uL (1mg of agarose --> 1uL)
2. 288uL

-->put in heat bath to melt (50 deg Celsius for ~10 mins; vortex every 2-3 mins?)
--> 1x vol of Isopropanol (original volume of gel)

1. 106uL
2. 96uL

-->Centrifuge at 13,000 rpm for 1 min; discard flowthrough
--> Add 500uL of QG
--> Centrifuge at 16,000 rpm for 1 min; discard flowthrough
--> Add 750uL of PE
--> Centrifuge again, I assume (not written in notes); dump PE
--> Dry run centrifuge
--> Add 50uL EB (elution buffer), let sit for 1 min (make sure to get it on the filter!)
--> Centrifuge; DO NOT discard flowthrough (this contains the DNA)
--> Nanodrop to measure concentration

Protip on pipets:

Try to work with 1 hand and not move the pipet much. Eg, take something, open with one hand, pipet things in, place it back down on the rack, etc.