...
"1-2_CMV_mKate" (1-2 is the destination vector, CMV is the promoter and mKate is the gene)
Running gel:
Check terminals (runs form +ve to -ve)
Fill with TAE till it covers the agarose gel (can leave the tray in)
Add dye (orange G)
--> 6x conc. so 8uL to 40uL (note, here the volume of dye is large enough to affect total conc.)
- Ladder
- 1 (15 uL) (for imaging) (1 = Left, EE)
- 2 (15 uL) (for imaging) (2 = Right, SS)
- Space
- 1 (~30 uL; fill as much as you can) (for extraction)
- 2 (~30 uL; fill as much as you can) (for extraction)
Wait till about halfway down ~30 mins
Extraction from gel:
- Image the gel
- Cut the extraction bands under UV (to see the bands)
- Follow protocol