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  1. VVS: GTG GTC TCC -> GTG GTG TCC
    1. GTC frequency: 0.24
    2. GTG frequency: 0.47

 

If you really care, you can mutate these sites back using site-directed mutagenesis (e.g. this kit: http://www.genomics.agilent.com/en/Site-Directed-Mutagenesis/QuikChange-Lightning/?cid=AG-PT-175&tabId=AG-PR-1162)