Page History

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1. (Kyle) TRE-LacOid_mKate in DEST 1-2
2. (Erik) Hef1a-lacO_ NLS-eYFP in DEST 1-2
3. (Erik) Hef1a-lacO_ eBFP in DEST 1-2
4. (Shinjini) CMV_mKATE in DEST 1-2
5. (Shinjini) CMV_eYFP in DEST 1-2

4/15/14

Transformations:

1. Put competent cells from -80 degree celcius freezer on ice
2. Add 2μL of LR mixture to the cells. Do NOT pipet up and down; swirl instead.
3. Keep cells on ice for 30 minutes.
4. Heat shock cells at 42  degrees celcius for EXACTLY 30 seconds
5. Put it on ice for 2 minutes.
6. The add .5mL (500μL) SOC 
7. Incubate at 37 degrees celcius for 1 hour. (Tape cell tubes together and label!)

Making SOC:

This is the ultra-broth for cell growth, so be extra careful not to get it contaminated.

1. 49mL of SOB
2. 1mL of 20% glucose.

Making Agar:

Follow instruction in pack.

Autoclave for around 20 minutes (+ ~20 minutes to pressurize and depressurize). Cool changing tape!