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Wiki Markup
Composition Setup
14-Mar-7
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LR 
 
 Reaction:
 Label 
 Gene 
 (fmol) 
 Promoter 
 (fmol) 
 Destinatioin 
 (fmol) 
 LR Clonase(uL) 
 Total Volume 
 JX 1 
 mKate 
 5 
 Het1a-LacO 
 5 
 1-2 
 10 
 1 
 4 
 JX 2 
 rtTA 
 5 
 Het1a 
 5 
 1-2 
 10 
 1 
 4 
14-Mar-14
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Gibson Reaction Mix
Sample Y166
 
 DNA 
 I-Sce-1 
 Buffer 
 Water 
 Total 
 Mass (μg) 
 1 
 
 
 
 
 Concentration (ng/μL) 
 316 
 
 
 
 
 Volume (μL) 
 3.16 
 1 
 2 
 13.84 
 20 
 Order 
 2 
 4 
 3 
 1 
 
Created Gibson reactio mix. Reactants entered in volume and order noted in table.
14-Mar-16
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Gibson Reaction Mix for All Samples
 Mass (μg) 
 Enzyme (μL) 
 Buffer (μL) 
 Total (μL) 
 
 1 
 1 
 2 
 20 
 
 
 
 
 
 
 
 Con (ng/μL) 
 Vol DNA (μL) 
 Vol H2O (μL) 
 
 1.1 
 151 
 6.62 
 10.38 
 CMU-Nde1 
 1.2 
 267 
 3.75 
 13.25 
 
 1.3 
 141 
 7.09 
 9.91 
 
 2.1 
 652 
 1.53 
 15.47 
 TRE-t-Sta1 
 2.2 
 263 
 3.80 
 13.20 
 
 2.3 
 212 
 4.72 
 12.28 
 
 4.1 
 149 
 6.71 
 10.29 
 TRE-t-Sta1 
 4.2 
 415 
 2.41 
 14.59 
 
 4.3 
 583 
 1.72 
 15.28 
 
 6.1 
 353 
 2.83 
 14.17 
 TRE-t-Sta1 
 6.2 
 593 
 1.69 
 15.31 
 
 6.3 
 361 
 2.77 
 14.23 
 
 7.1 
 448 
 2.23 
 14.77 
 Het1a-Xhol 
 7.2 
 237 
 4.22 
 12.78 
 
 7.3 
 186 
 5.38 
 11.62 
 
 8.1 
 246 
 4.07 
 12.93 
 Het1a-Lac-BamH1 
 8.2 
 182 
 5.49 
 11.51 
 
 8.3 
 871 
 1.15 
 15.85 
 
 9.1 
 410 
 2.44 
 14.56 
 TRE-t-Sta1 
 9.2 
 214 
 4.67 
 12.33 
 
 9.3 
 185 
 5.41 
 11.59 
 
 10.1 
 285 
 3.51 
 13.49 
 CMU-Nde1 
 10.2 
 547 
 1.83 
 15.17 
 
 10.3 
 388 
 2.58 
 14.42 
 

|| Label \\ || Gene \\ || (fmol) \\ || Promoter \\ || (fmol) \\ || Destinatioin \\ || (fmol) \\ || LR Clonase(uL) || Total Volume \\ ||
| JX 1 \\ | mKate \\ | 5 \\ | Het1a-LacO \\ | 5 \\ | 1-2 \\ | 10 \\ | 1 \\ | 4 \\ |
| JX 2 \\ | rtTA \\ | 5 \\ | Het1a \\ | 5 \\ | 1-2 \\ | 10 \\ | 1 \\ | 4 \\ |
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14-Mar-14

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Gibson Reaction Mix

Sample Y166
| | DNA | I-Sce-1 | Buffer | Water | Total |
| Mass (μg) | 1 | | | | |
| Concentration   (ng/μL) | 316 | | | | |
| Volume (μL) | 3.16 | 1 | 2 | 13.84 | 20 |
| Order | 2 | 4 | 3 | 1 | |
Created Gibson reactio mix. Reactants entered in volume and order noted in table.

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14-Mar-16

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Gibson Reaction Mix for All Samples

| Mass (μg) | Enzyme (μL) | Buffer (μL) | Total (μL) | |
| 1 | 1 | 2 | 20 | |
| | | | | |
| | Con (ng/μL) | Vol DNA (μL) | Vol H2O (μL) | |
| 1.1 | 151 | 6.62 | 10.38 | CMU-Nde1 |
| 1.2 | 267 | 3.75 | 13.25 | |
| 1.3 | 141 | 7.09 | 9.91 | |
| 2.1 | 652 | 1.53 | 15.47 | TRE-t-Sta1 |
| 2.2 | 263 | 3.80 | 13.20 | |
| 2.3 | 212 | 4.72 | 12.28 | |
| 4.1 | 149 | 6.71 | 10.29 | TRE-t-Sta1 |
| 4.2 | 415 | 2.41 | 14.59 | |
| 4.3 | 583 | 1.72 | 15.28 | |
| 6.1 | 353 | 2.83 | 14.17 | TRE-t-Sta1 |
| 6.2 | 593 | 1.69 | 15.31 | |
| 6.3 | 361 | 2.77 | 14.23 | |
| 7.1 | 448 | 2.23 | 14.77 | Het1a-Xhol |
| 7.2 | 237 | 4.22 | 12.78 | |
| 7.3 | 186 | 5.38 | 11.62 | |
| 8.1 | 246 | 4.07 | 12.93 | Het1a-Lac-BamH1 |
| 8.2 | 182 | 5.49 | 11.51 | |
| 8.3 | 871 | 1.15 | 15.85 | |
| 9.1 | 410 | 2.44 | 14.56 | TRE-t-Sta1 |
| 9.2 | 214 | 4.67 | 12.33 | |
| 9.3 | 185 | 5.41 | 11.59 | |
| 10.1 | 285 | 3.51 | 13.49 | CMU-Nde1 |
| 10.2 | 547 | 1.83 | 15.17 | |
| 10.3 | 388 | 2.58 | 14.42 | |
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Gel 
 
 Figure 
 
 for 
 
 Previous 
 
 Samples:



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