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Sunrise Yeast Nitrogen Base (YNB) contains salts, vitamins and trace elements as defined: Potassium phosphate (1000 mg/L), Inositol (2 mg/L), Copper sulfate (0.04 mg/L), Magnesium sulfate (500 mg/L), Niacin (0.4 mg/L), Potassium iodide (0.1 mg/L), Sodium chloride (100 mg/L), PABA (0.2 mg/L), Ferric chloride (0.2 mg/L), Calcium chloride (100 mg/L), Pyridoxine (0.4 mg/L), Manganese sulfate (0.4 mg/L), Biotin (0.002 mg/L), Riboflavin (0.2 mg/L), Sodium molybdate (0.2 mg/L), Pantothenate, calcium (0.4 mg/L), Thiamine (0.4 mg/L), Zinc sulfate (0.4 mg/L), Folic acid (0.002 mg/L), Boric acid (0.5 mg/L).
Synthetic Dropout Media Recipe (if you are using Sunrise's YNB + Nitrogen bottle)
Name | Final Concentration | For 500 mL Volume |
|---|---|---|
Yeast Nitrogen Base + Ammonium Sulfate (YNB + Nitrogen) | 6.7 g/L | 3.35 g |
Amino acid (AA) mix (CSM-AA) | As noted on the container | x g |
ddH2O |
| 450 mL |
Yeast Agar (for plates only) | 20 g/L | 10 g |
AUTOCLAVE AND THEN COOL, THEN ADD... |
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Sterile dextrose (20% w/v stock concentration) | 2% w/v | 50 mL |
Synthetic Dropout Media Recipe (if you are using Sunrise's YNB bottle):
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