Page History

Performed two LR reactions for practice

Aliquot 1:

1 uL of eYFP @ 5 fm/uL

1 uL of TRE-t @ 5fm/uL

1 uL of DEST @ 10 fm/uL

1 uL of clonase

total: 4 uL

Aliquot 2:

1 uL of eYFP @ 5 fm/uL

1 uL of HEF-1a @ 5fm/uL

1 uL of DEST @ 10 fm/uL

1 uL of clonase

let sit at room temperature overnight