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- Agencourt Ampure XP, A63881 (60mL, $300)
- 2 Roche LichtCycler480 384-well plate
- 1:100 dilution of SYBR stock (Invitrogen S7563, 10,000x)
- Step 1/ Initial QPCR Primers ( PE_16s_v4U515-F)
- Step 2 primers ( PE-III-PCR-F, PE-IV-PCR-XXX; NOTE - PE-IV-PCR-XXX primers should be used as a pre-pipetted 96well plate)
- Final QPCR primers (BMC Final F, BMC Final R)
- HF Phusion (NEB, M0530L)
- KAPA SYBR 2xMM for final QPCR (can be purchased from the BMC)
- Invitrogen Super magnet (16 or 8 sample capacity)
- 96 well magnet plate
- X up to 10 boxes of 300uL filtered epTIPS (Cat#XXEppendorf, Cat# 0030014456, reloads Cat# 0030014472)
- X up to 13 boxes of 300uL unfiltered epTIPS (Cat#XXepTIPS (Eppendorf, bulk Cat# 960050620)
- X 8 boxes of 50uL filtere epTIPS (Cat#XXfiltered epTIPS (Eppendorf, Cat# 0030014413, reloads Cat# 0030014430)
- up to 10 30mL reagent reservoirs (Eppendorf, 250 reservoirs Cat# 960051500)
- up to 2 100mL reagent reservoirs (Eppendorf, 250 reservoirs Cat# 960051511)
Determination of Step 1 Cycle Time and Sample Check:
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