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Materials:
● Agencourt Ampure XP, A63881 (60mL, $300)
● 2 Roche LichtCycler480 384-well plate
● 1:100 dilution of SYBR stock (Invitrogen S7563, 10,000x)
● Step 1/ Initial QPCR Primers ( PE_16s_v4U515-F)
● Step 2 primers ( PE-III-PCR-F, PE-IV-PCR-XXX)
● Final QPCR primers (PE Seq BMC Adapter F, PE Seq BMC Adapter R)
● HF Phusion (NEB, M0530L)
● KAPA SYBR 2xMM for final QPCR
● Invitrogen Super magnet (16 or 8 sample capacity)
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Reagents | X1 RXN (uL) |
H2O | 7.2 |
PE Seq Primer BMC Adapter – F (10uM) | 0.4 |
PE Seq Primer BMC Adapter – R (10uM) | 0.4 |
KAPA SYBRgreen MM | 10 |
Template | 2 |
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