...
SPRI Clean Up
Materials used:
○ SPRI beads
○ 70% EtOH
○ EB
○ Invitrogen super magnet
- Vortex cold AmpureXP beads, pool DNA from PCR tubes (~100uL)
- Aliquot 85.5uL beads into Epi’s – let equilibrate to RT
- Add DNA (take 95uL) + beads (85.5uL) = 180.5 uL
- Incubate 13’ 13 mins @ RT
- Separate ON magnet 2’2 mins
- While ON magnet, remove/discard SN
- Wash beads 2x with 70% EtOH, 500uL each wash
- Air dry beads for 15-20’ 20mins on magnet
- Remove from magnet, elute in 40uL H2O, vortex to resuspend
- Incubate (at least 7’)7 mins @ RT
- Separate on magnet 2’2mins
- Collect 35-40 ul and save SN
...
SPRI Clean Up
Materials used:
○ SPRI beads
○ 70% EtOH
○ EB
○ Invitrogen super magnet
- Vortex cold AmpureXP beads, pool DNA from PCR tubes (~100uL)
- Aliquot 85.5uL beads into Epi’s – let equilibrate to RT
- Add DNA (take 95uL) + beads (85.5uL) = 180.5 uL
- Incubate 13’ 13mins @ RT
- Separate ON magnet 2’2mins
- While ON magnet, remove/discard SN
- Wash beads 2x with 70% EtOH, 500uL each wash
- Air dry beads for 15-20’ 20mins on magnet
- Remove from magnet, elute in 40uL H2O, vortex to resuspend
- Incubate (at least 7’)7mins @ RT
- Separate on magnet 2’2mins
- Collect 35-40 ul and save SN
...