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1. Grow the appropriate host strain (BL21 for wildtype or T7-415) overnight in M9TB media. Next morning, inoculate around 100 uL of the host strain in 4mL of M9TB and grow to an OD of 1.0 at 37 degrees (for BL21 this takes between 2-3 hrs). This The cells can be stored at 4 degrees until ready for use. Do not store for more than 2 days.
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