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Name | Final Concentration | For 500 mL Volume |
|---|---|---|
Yeast Nitrogen Base (YNB) | 1.7 g/L | 0.85 g |
Ammonium Sulfate | 5 g/L | 2.5 g |
Amino acid (AA) mix (CSM-AA) | As noted on the container | x g |
ddH2O |
| 450 mL |
Agar (for plates only) | 20 g/L | 10 g |
AUTOCLAVE AND THEN COOL, THEN ADD... |
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Sterile dextrose (20% w/v stock concentration) | 2% w/v | 50 mL |
CSM mix for making drop out aa mix:
CSM Component | mg/L (1x) | g/L (100X) |
Adenine | 10 | 1 |
L-Alanine | 0 | 0 |
L-Arginine | 50 | 5 |
L-Asparagine | 0 | 0 |
L-Aspartic Acid | 80 | 8 |
L-Cysteine | 0 | 0 |
Glutamine | 0 | 0 |
L-Glutamic Acid | 0 | 0 |
Glycine | 0 | 0 |
L-Histidine | 20 | 2 |
Myo-Inositol | 0 | 0 |
L-Isoleucine | 50 | 5 |
L-Leucine | 100 | 10 |
L-Lysine | 50 | 5 |
L-Methionine | 20 | 2 |
Para-AminoBenzoic Acid (PABA) | 0 | 0 |
L-Phenylalenine | 50 | 5 |
L-Proline | 0 | 0 |
L-Serine | 0 | 0 |
L-Threonine | 100 | 10 |
L-Tryptophan | 50 | 5 |
L-Tyrosine | 50 | 5 |
Uracil | 20 | 2 |
L-Valine | 140 | 14 |
Remove AAs from this mix to make corresponding drop out mixes (e.g. to make -Ura-Trp drop out, add all the above AAs except Ura and Trp)
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