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12. Centrifuge the tubes at 13000g for 30 seconds and remove the supernatant.
A. *For prototrophic gene selection: Pipette Pipette 200 uL - 1.0 ml of sterile water into the transformation tube. Stir the pellet with a sterile micropipette tip to break the cell pellet and then vortex mix to uniformly resuspend pellet. **{} I usually resuspend in 200 uL.*
B. *For eukaryotic antibiotic gene selection: *Pipette Pipette 200 uL - 1.0 ml of YPD liquid medium into the transformation tube. Vortex mix to resuspend pellet. Incubate for 2--3 h at 30C to ensure good expression from the input plasmid DNA.
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