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1 gm of a solute in 100 ml of a solvent makes a 1% (w/v) solution. Hence, if you are pouring a 2% gel in to the small gel box, you want to use 1.2 gm of the gel powder.
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4. Let the box aside (or place in the refrigerator) to cool for about an hour or so until it turns in to an opaque gel.
5. After the gel solidifies, fill the gel box with TAE solution until the gel is submerged.
6. While loading the gel, remember that the loading buffer is 6x. Hence, you want to mix 10 ul for a 50 ul sample to load on to a 60 ul well (A well from a 1.5 comb can hold 60 ul of sample).
67. For the 2 log ladder, use 10 ul of the the NEB ladder.
78. Run the gel at constant voltage around 175 V for > 20 min.
Warning: 175V can make the gel hot and warp your bands. If this is concern, use a lower voltage.