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Expand
titleGolden Gate Parts
  1.  TEVp PCR
  2. tTEVp PCR
  3. Syk PCR
  4. Q3-RFP-Q4
  5. Q8-12-Q6
  6. Q8-3-Q6
  7. Q8-9-Q6
  8. Q8-6-Q6
  9. 3-TCS
  10. 6-TCS
  11. 9-TCS
  12. 12-TCS
  13. 15-TCS
  14. TCS-3
  15. TCS-6
  16. TCS-9
  17. TCS-12
  18. TCS-15
  19. GG DONR
  20. GG DONR

Expand
titleLR Parts
  1.  Syk-eYFP
  2. Gmab Heavy
  3. pENTR 6 (Velia)
  4. pENTR 10 (Velia)
  5. Lyn-TEVp (iGEM)
  6. Gmab-Light
  7. Lyn
  8. Syk-eYFP
  9. Syk
  10. mKate
  11. Lyn-mKate
  12. CD79A-TCS-Gal4VP16 (iGEM)
  13. CD79A
  14. hEF1a-LacO:
  15. TRE:
  16. Gal4VP16
  17. hEF1a:
  18. LacI
  19. Syk-TEV (iGEM)
  20. CMV5-CuO:
  21. hEF1a-CuO:
  22. CymR
  23. pDEST 1-2
  24. pDEST 2-3
  25. pDEST 4-5
  26. CD79B
Expand
titlepEXPRs
  1.  hEF1a:CD79B-pTet:RFP-Gal4VP16
  2. UAS:eYFP
  3. pEXPR 106 (Velia)
  4. pEXPR 8 (Velia)
  5. hEF1a:CD79A-TCS-Gal4VP16
  6. TRE:Syk-TEV (iGEM)
  7. hEF1a:LacI
  8. hEF1a:mKate
  9. TRE:CD79B
  10. hEF1a:Gal4VP16
  11. hEF1a:Gmab Light
  12. TRE:CD79B
  13. TRE:CD79A
  14. TRE:Gmab Light
  15. hEF1a:Gmab Heavy
  16. hEF1a:eBFP
  17. pLac:mKate (pLac = hEF1a-LacO)
  18. TRE:Gal4VP16
Expand
titleUndiluted Midis
  1.  hEF1a:Gal4VP16
  2. hEF1a:CD79B-TCS-Gal4VP16 (iGEM)
  3. UAS:eYFP
  4. hEF1a:mKate
  5. TRE:Gal4VP16
  6. hEF1a:rtTA
  7. hEF1a-LacO:mKate (pLac: MKate is the same thing)
  8. TRE:mKate
  9. hEF1a:LacI
  10. UAS:mKate
  11. hEF1a:CD79A
  12. hEF1a:CD79B
  13. hEF1a:Lyn
  14. hEF1a:GMab Heavy
  15. hEF1a:GMab Light
  16. hEF1a:CD79A-TCS-Gal4VP16 (iGEM)
  17. TRE:Syk-TEVp (iGEM)
Expand
titleTC Working Midis
  1. hEF1a:rtTA
  2. hEF1a:mKate
  3. hEF1a:eBFP
  4. UAS:eYFP
  5. TRE:Gal4VP16
Expand
titleUndiluted, Unannealed Linkers
  1. 9t9f
  2. 3t15f
  3. 6t12f
  4. 12t6f
  5. 15t3f
  6. 3TCSF
  7. 3TCSR
  8. 6TCSF
  9. 6TCSR
  10. 9TCSF
  11. 9TCSR
  12. 12TCSF
  13. 12TCSR
  14. 15TCSF
  15. 15TCSR
  16. TCS3F
  17. TCS3R
  18. TCS6F
  19. TCS6R
  20. TCS9F
  21. TCS9R
  22. TCS12F
  23. TCS12R
  24. TCS15F
  25. TCS15R
  26. 3AAF
  27. 3AAR
  28. 6AAF
  29. 6AAR
  30. 9AAF
  31. 9AAR
  32. 12AAF
  33. 12AAR

Expand
titleSequencing Primers
  1.  CD79A-TCS-Gal4VP16 S1
  2.  CD79A-TCS-Gal4VP16 S2
  3.  CD79A-TCS-Gal4VP16 S3
  4.  CD79A-TCS-Gal4VP16 S4
  5. CD79B-TCS-Gal4VP16 S1
  6. CD79B-TCS-Gal4VP16 S2
  7. CD79B-TCS-Gal4VP16 S3
  8. CD79B-TCS-Gal4VP16 S4
  9. TRE:Syk-RFP-TEVp S1
  10. TRE:Syk-RFP-TEVp S2
  11. TRE:Syk-RFP-TEVp S3
  12. TRE:Gal4VP16 S1
  13. TRE:Gal4VP16 S2
Expand
titlePCR/SDM Primers
  1.  CD79B SDM F1
  2. CD79B SDM R1
  3. CD79B SDM F2
  4. CD79B SDM R2
  5. CD79B SDM F3
  6. CD79B SDM R3
  7. Gal4VP16 SDM F4/5 (Origionally labelled CD79B SDM F5, in some cases labelled 4 instead of 5 still same thing)
  8. Gal4VP16 SDM R4/5 (Origionally labelled CD79B SDM R5, in some cases labelled 4 instead of 5 still same thing)
  9. Syk F1
  10. Syk R1
  11. TEV F1
  12. TEV R1
  13. tTEV F1
  14. tTEV R1
  15. CD79B F3
  16. CD79B R3