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14-8-7

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pENTR-L1-TALER14-L2 Digest AseI 3.1

 

 

 

 

14-8-8

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LR Gateway

MAV1212-RFP
hEF1a
TAL14

MAV1212-RFP
hEF1a
TAL21

 

 

 

14-8-11

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Transformation

MAV1212-hEF1a-TALER14

MAV1212-hEF1a-TALER21

 

 

14-8-15

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I dont understand i dont know i dont

 

 

14-8-18

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LR
MAV1212-mRFP
pENTR-L4-hEF1a-R1
pENTR-L1-TALER14-L2

 

 

14-8-19

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Transformation
MAV1212-hEF1a-TALER14

 

 

14-8-20

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PropagationLR
MAV1212-hEF1a-TALER14

MAV1212-mRFP

pENTR-L4-hEF1a-R1

pENTR-L1-TALER14-L2

 

 

14-8-21

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DigestTransformation
MAV1212-hEF1a-TALER14:MAV1212-hEF1a-TALER14
DNACon (ng/µL)EnzymeBufferVol DNAMass DNAVol EnzymeVol BufferVol WaterVol Total
Ladder         
TAL14 Insert 1282.5PstI3.13.5410001213.4620
TAL14 Insert 2316.9PstI3.13.1610001213.8420
TAL14 Control316.9  3.161000  16.8420