Page History

JX 1

mKate

5

Het1a-LacO

5

1-2

10

1

4

JX 2

rtTA

5

Het1a

5

1-2

10

1

4

DNA

I-Sce-1

Buffer

Water

Total

Mass (μg)

1

Concentration (ng/μL)

316

Volume (μL)

3.16

1

2

13.84

20

Order

2

4

3

1

Mass (μg)

Enzyme (μL)

Buffer (μL)

Total (μL)

1

1

2

20

Con (ng/μL)

Vol DNA (μL)

Vol H2O (μL)

1.1

151

6.62

10.38

CMU-Nde1

1.2

267

3.75

13.25

1.3

141

7.09

9.91

2.1

652

1.53

15.47

TRE-t-Sta1

2.2

263

3.80

13.20

2.3

212

4.72

12.28

4.1

149

6.71

10.29

TRE-t-Sta1

4.2

415

2.41

14.59

4.3

583

1.72

15.28

6.1

353

2.83

14.17

TRE-t-Sta1

6.2

593

1.69

15.31

6.3

361

2.77

14.23

7.1

448

2.23

14.77

Het1a-Xhol

7.2

237

4.22

12.78

7.3

186

5.38

11.62

8.1

246

4.07

12.93

Het1a-Lac-BamH1

8.2

182

5.49

11.51

8.3

871

1.15

15.85

9.1

410

2.44

14.56

TRE-t-Sta1

9.2

214

4.67

12.33

9.3

185

5.41

11.59

10.1

285

3.51

13.49

CMU-Nde1

10.2

547

1.83

15.17

10.3

388

2.58

14.42