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| Untransfected | Single Color Blue | Background anti-IgM binding | Single Color Red | Double Color | -VE control (background activation) | -VE control (background cleavage) | +VE control | |
|---|---|---|---|---|---|---|---|---|
| HEK293 | HEK293 hEF1a:CymR CuO:MabH CuO:MabL hEF1a:Lyn
CuO:CD79A-15TCS1512TCS12-G4VP16 CuO:CD79B TRE:Syk-15112-tTEVp Dox 1 nM Cumate 500uM | HEK293 hEF1a:CymR CuO:MabH CuO:MabL hEF1a:Lyn
CuO:CD79A-15TCS1512TCS12-G4VP16 CuO:CD79B TRE:Syk-1512-tTEVp Dox 1 nM Cumate 500uM anti-IgM 1uL/100uL | HEK293 hEF1a:CymR CuO:MabH CuO:MabL hEF1a:Lyn
CuO:CD79A-15TCS1512TCS12-G4VP16 CuO:CD79B TRE:Syk-1512-tTEVp Dox 1 nM Cumate 500uM | HEK293 hEF1a:CymR CuO:MabH CuO:MabL hEF1a:Lyn CuO:CD79A-15TCS1512TCS12-G4VP16 CuO:CD79B TRE:Syk-1512-tTEVp Dox 1 nM Cumate 500uM | HEK293 hEF1a:CymR CuO:MabH CuO:MabL hEF1a:Lyn UAS:mKate CuO:CD79A
CuO:CD79B TRE:Syk-1512-tTEVp Dox 1 nM Cumate 500uM | HEK293 hEF1a:CymR CuO:MabH CuO:MabL hEF1a:Lyn UAS:mKate CuO:CD79A- | 15TCS1512TCS12-G4VP16 CuO:CD79B TRE:Syk
Dox 1 nM Cumate 500uM | HEK293 hEF1a:CymR CuO:MabH CuO:MabL hEF1a:Lyn UAS:mKate CuO:CD79A
CuO:CD79B TRE:Syk-1512-tTEVp hEF1a:GAl4VP16 Dox 1 nM Cumate 500uM |
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