Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

Thursday's to do's 

•  Midis:
  •  B9T12
  •  B15T3
  •  S9TEV
  •  S12TEV
•  Minis, sequencing 
  •  B6T9
  •  A9T15
•  Inoculate mini
  •  B15T9
  •  B15T15
•  Inoculate midi:
•  Sequencing:
  •  B6T9-1
  •  A9T15-2
•  Restreak:
  •  B15T9

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Check on status:

• B9T12 - send for seq
• B6T9 - send for seq
• B12T9 - repeat seq
• B12T12 - repeat seq

Non-Lab To Do's 

•  Cytometry time 
•  Order Cumate 
•  Chicken lysozyme, gp120
•  Antibodies for antigen binding detection

Waiting on primers to do list:

Higher Level To Do's:

•  Decide on other antigen-variable regions we want to test  
•  Is gantenerumab proprietary and can we actually publish with it?

THINGS TO NOT FORGET TO DO:

• SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
• 3T3 MIDI