| gel lane | 0 | 1 | 2 |
| DNA construct | Constants Definition | pENTR HyHEL-10-H | pENTR HyHEL-10-H |
| Tube identifier | 1 | 2 | |
| DNA concentration (ng/ul) | 223 | 117 | |
| Enzyme Name | BbsI | BbsI | |
| Buffer Name | 2.1 | 2.1 | |
| strip tube # | 1/1 | 1/2 | |
| water volume | 17.0 | 14.8 | 12.7 |
| DNA volume | 2.24 | 4.27 | |
| buffer volume | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 |
| loading dye | 4 | 4 | 4 |
| DNA mass desired (ng) | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 |
| gel/lane | #REF! | 1 | 2 |
| results | |||
| notes | |||
| virtual gel |