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Construct
pENTR HyHEL-10 Heavy
pENTR VRC01 Heavy
pENTR VRC01 Light
CD79A3t3    
EnzymeBbsIBsrGI NcoI

 

   
Tube/Lane 12345   
Virtual gel Image RemovedImage RemovedImage RemovedImage Removed   

Note:  we were out of p200 tips, so I used half volumes so I only had to use p10s

...

pENTR VRC01 Heavy
pENTR HyHEL-10 Heavy
CD79A3t3
EnzymeApaIBsrGIBbsINcoI
Tube/Lane 1,23,45,67
Virtual gel Image AddedImage AddedImage AddedImage Added

Image Added

...