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ConstructA-6T9 midiA-9T9 midiA-9T15 midiA-15T3 midiA-15T15 midihEF1a:Gal4 midi
EnzymeNcoINcoINcoINcoINcoI 

BsrGI, StuI

Cut in hEF1a and Gal4

Tube/Lane 123456
Virtual gel      Image Added
ConstructA-3T15 miniA-12T9 miniA-15T9 mini
EnzymeNcoINcoINcoI
Tube/Lane 1, 23, 45, 6
Virtual gel