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Broken constructs

TRE:Syk-15-tTEV

  • PCR Syk-15-tTEV from TRE:Syk-15-TEV
  • Golden gate into pENTR (AarI)
  • LR with TRE

hEF1a:CD79B-RFP-G4

  • PCR RFP-G4 from hEF1a:CD79A-RFP-G4
  • PCR CD79B from hEF1a:CD79B-TCS-G4
  • Golden gate into pENTR (AarI)
  • LR with hEF1a

TRE:Syk-linker-tTEV