...
| Construct | hEF1a:CD79A-6T9-Gal4VP16 | hEF1a:CD79A-9T12-Gal4VP16 | hEF1a:CD79A-9T15-Gal4VP16 | hEF1a:CD79A-12T9-Gal4VP16 | hEF1a:CD79A-12T12-Gal4VP16 | GG Donr | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Lane | 1 - #1 2 - #2 | 3 | 4 | 5 - #1 6 - #2 | 7 - #1 8 - #2 | 9 - NEB T4 ligase buffer 10 - Promega ligase buffer | |||||
| Enzyme | NcoI | NcoI | NcoI | NcoI | NcoI | AarI | |||||
| Virtual Gel |  |  | |||||||||
| Conclusion |
...