Tuesday's To Do's
- Gel for oligo annealing
- Mix new annealing buffer
- Anneal oligos
- Design IgM Sequencing Primers
- CD79B, gal4 PCR
- Golden Gates:
- hEF1a:CD79B-pTET:mRFP-Gal4 golden gate
- TRE:Syk-15-tTEVp
- Transform
- hEF1a:CD79B-pTET:mRFP-Gal4 GG
- TRE:Syk-15-tTEVp GG
- TRE:Syk-pTET:mRFP-tTEVp sequencing
- Pick pENTR RFP-IgM Colony
Wednesday's to Dos
- rePCR Gal4, CD79B
- Gel PCRs
- GG hEF1a:CD79B-pTet:RFP-Gal4VP16
- Transform hEF1a:CD79B-pTet:RFP-Gal4VP16
Thursday's to Dos
- Mini pENTR Syk-15-tTev
- hEF1a:Gal4 pick new colony
- pick hEF1a:CD79B-RFP-Gal4VP16 colonies
- Innoculate midis of CDA linker
- Pick colonies of failed CDA linker constructs
- Analyze TRE:Syk-pTet:RFP-Gal4vp16 sequencing
Sequencing account information:
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Non-Lab To Do's
- Cytometry time
- Order Cumate
- Chicken lysozyme, gp120
- Antibodies for antigen binding detection
Waiting on primers to do list:
- RFP-IgM sequencing
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?