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So far, only checked for correct linker insertion. + = good, -- = not good, ? = unsure

 3-6-9-12-15-
-3--(minus) +(plus) +(plus)
-6 +(plus)+(plus)+(plus) 
-9+(plus)--(minus)+(plus)--(minus)--(minus)
-12 +(plus)?(question)--(minus) 
-15--(minus) --(minus) +(plus)

 

 

 

 

 

 

Possible missense mutations in Gal4 of 9T9 and 15T15 - Geneious says it's a mutation but the trace is not great at these points.  9T12 is a bad read after the linker.

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