Thursday's To Do's
- Innoculate midi cultures:
- hEF1a:tagBFP
- hEF1a:gal4VP16
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-Gal4vp16
Wednesday's To Do's
- Transform
- CD79A linker golden gates
- TRE:Syk-15-tTEVp golden gate
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:gal4vp16
- TRE:mKate
- hEF1a:CD79B-TCS-Gal4vp16
- Design additional Syk-TEV Sequencing Primer
- order primers to re-pcr CD79B-RFP-G4
- Antibody PCRs
- Purify
- Gel
- Golden Gate
- Non-Lab To Do's:
- Order chicken lysozyme and gp120?
- order antibodies for antigen binding detection
Geneious To Do List:
- Antibody cloning for chicken lysozyme - Kathryn
-
hef1a sequencing primersBCR 2.0 To Do
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?