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 hEF1a:CymRhEF1a-CuO:mKateTRE:SykEGSH:mKate

hEf1a:VgEcR-2A-RXR

TRE:mKatehEF1a:Gal4VP16hEF1a:CD79B-TCS-Gal4VP16hEF1a:tagBFP
Enzyme

PstI

Cuts in hEF1a, CymR and backbone

StuI

Cuts in hEF1a, mKate and backbone

StuI, ApaI

Cuts in TRE, Syk and backbone

  

ApaI

Cuts in hEF1a, VgEcR-2A-RxR and bacbone

StuI

cuts in TRE, mKate, and backbone

XhoI

cuts in hEF1a, Gal4VP16, and backbone

PvuII

Cuts in CD79B, TCS and backbone

 
Virtual gel  Image Added