Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.
2015-07-10
Monday's To Do's 
  •  Sodium Azide Solution
  •  Make Kan X-gal plates (restart) MidiMidi's:
  •  digest, gel: transform
  •  Make Sodium Azide solution 
  •  Pick colonies:
    •  TRE:Syk-15-tTEVp golden gate
    •  Golden gate donor mini product
    •  LRs:
      •  EGSH:mKate
      •  hEf1a:VgEcR-2A-RXR
      •  TRE:mKate
      •  hEF1a:Gal4VP16 (remember which pDEST)
      •  hEF1a:CD79B-TCS-Gal4VP16 
      •  hEF1a:tagBFP

Expand

titleOld To Do
Datestuff2015-07-09
  •  gel verify:
  •  Pick colonies for midi
  •  PCR purify TRE:Syk-15-tTEVp
  •  Golden gate TRE:Syk-15-tTEVp
  •  LRs:
    •  EGSH:mKate
    •  hEf1a:VgEcR-2A-RXR
    •  TRE:mKate
    •  hEF1a:Gal4VP16 (remember which pDEST)
    •  hEF1a:CD79B-TCS-Gal4VP16 
    •  hEF1a:tagBFP
2015-07-08

Non-Lab To Do's:

  •  Flow cytometry with Brian (big grin)
  •  Order chicken lysozyme and gp120?
  •  
Non-lab to do's
  •  
    •  Design (or find?) sequencing primers for mIgM CH (constant region)
    •  Plan experiment to test gantenerumab binding to beta-amyloid
    •  Leader sequences for ordering gBlocks of variable regions?

CMV5-CuO 715:

hEF1a-CuO 715:09

CymR-CuO 710:09

  • order antibodies for antigen binding detection

 

Geneious To Do List:
  •  Antibody cloning for chicken lysozyme - Kathryn 
  •  hef1a sequencing primersBCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

 

 

Higher Level To Do's:
  •  Decide on other antigen-variable regions we want to test  
  •  Other experimental planning (on experimental page)
  •  Parts list (excel sheet detailing specs/locations of parts)
  •  How do we do site directed mutagenesis?
  •  Literature research: 
    •  kozak sequences for heavy and light chains and CD79s
    •  truncated TEVp
    •  other antigens to detect using BCR (we were thinking of small molecule detection)
    •  inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first) 
  •  Cloning planning 
    •  hEF1a:Syk-pTet:mRFP-TEVp
    •  variable region 
  •  Is gantenerumab proprietary and can we actually publish with it?